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. 2019 Jun 26;20(13):3113. doi: 10.3390/ijms20133113

Figure 3.

Figure 3

Colocalization of desmogleins and flotillin-2 upon 2 mM calcium in hTert cells. The cells were grown on coverslips in KGM with 0.05 mM calcium for at least four days, and then shifted to 2 mM calcium for 24 h. After MeOH fixation, the cells were stained with anti-Dsg3 (green) and anti-Flot2 (red) antibodies and fluorochrome coupled secondary antibodies (anti-mouse Alexa488 and anti-rabbit Alexa546). The coverslips were mounted in a mounting medium with DAPI. n = 3 independent experiments, scale bar 20 µm.