Figure 3.

Worms deficient in mitochondrial fission and fusion are protected from 6-OHDA-induced neurodegeneration. For experiments, synchronized L1 larvae were provided with food and allowed to develop to the L4 stage, which was 48 h in wild-type and drp-1 mutants and 72 h in slow-growing fzo-1 mutants (Panel A). L4 worms were then exposed to 6-OHDA for 1 h, and neurodegeneration was measured 48 h (Panel B) and six days (Panel C) following exposure. Scoring was performed as described in Methods: 0 represents no degeneration, while increasing numbers represent increasing severity of degeneration. Data shown are combined from three biological replicates with each treatment group comprising n > 40 worms scored for dendrites on each of 4 cephalic neurons for a total of >120 dendrites scored.