Figure 6.
Antioxidant gene expression is not significantly altered in pink-1 and pdr-1 mutants. For experiments, wild-type, pink-1 mutant, and pdr-1 mutant worms expressing the Pdat-1::GFP transgene were exposed to 6-OHDA (grey bars) or solvent (white bars) for one hour as young adults (similar to the exposure scheme shown in Figure 5, Panel A). RNA was isolated 24 h following exposure, and mRNA of catalase (ctl-2, panel A), glutathione S-transferase (gst-10, panel B), and thioredoxin (trx-1, panel C) was measured using qPCR as described in Methods. Asterisks represent significance (p < 0.05) compared to the non-exposed control of the same strain in a two-way ANOVA with Sidak’s multiple comparison test.