Table 1.
Phosphoproteomics revealed a cluster of dysregulations (downregulations in blue, upregulations in red color) in factors associated with microtubules and microfilaments. The list also presents signal intensity and coefficient of variance (CV), the phosphorylation site, the peptide sequence around the phosphorylated residue (illustrated by asterisk *), and the peptide count. A selective massive deficit of pT1928 in microtubule-associated protein 1B (MAP1B) and the second strongest deficit of pS3781 in ANK2 as a MAP1B-interactor were documented. These prominent changes clustered with an enrichment of factors that link the cytoskeleton to either the mitochondria or to the plasma membrane signaling apparatus, namely MAP2, EPB41L2 (4.1G), MARK1, MAP1A, KIF1A, RP1, ADD2, CLASP1, and EPB41L1 (4.1N), see Figure 3A.
| Normalized Fold Change | Max % CV | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| DM all: Control | DM 1: Control | DM 2: Control | DM 3: Control | Max Intensity | Biological | Gene Name | Protein Name | Site | Peptide | Count in Details |
| −314.3 | −255.3 | −438.7 | −279.5 | 6,559,198 | 35.4 | Mtap1b | MAP1B | §1928 | TIKT*PEDGGYTCEITEK | 16 |
| −3.3 | −543.3 | −715.4 | −1.1 | 858,179 | 155.1 | Ank2 | ANK2 iso2 + iso3 | §3781; §3809; §872 | GSPIVQEPEEASEPKEESS*PRK | 29 |
| −3.0 | -1.0 | −47.8 | −31.0 | 947,741 | 143.6 | Vps13d | VPS13D | §2429 | NAS*SESAVVPK | 8 |
| −2.3 | −2.9 | −2.9 | −1.6 | 26,223 | 54.5 | Mtap2 | MAP2 iso6 | §1801; §471 | RLSNVSSS*GSINLLESPQLATLAEDVTAALAK | 3 |
| −2.3 | −2.9 | −2.9 | −1.6 | 26,223 | 54.5 | Mtap2 | MAP2 iso6 | §1803; §473 | RLSNVSSSGS*INLLESPQLATLAEDVTAALAK | 3 |
| −2.1 | −2.1 | −3.1 | −1.4 | 436,205 | 32.6 | Epb4.1l2 | EPB41L2 iso3 | §86 | QRS*YNLVVAK | 3 |
| −1.9 | −4.5 | −1.5 | −1.4 | 543,218 | 47.1 | Dapk2 | DAPK2 | §299 | RES*VVNLENFKK | 11 |
| −1.8 | −2.2 | −1.4 | −2.0 | 27,656 | 33.9 | Mark1 | MARK1 | §394, §403 | SRPSS*DLNNSTLQS*PAHLK | 19 |
| −1.7 | −2.1 | −1.7 | −1.4 | 405,747 | 33.8 | Vps13c | VPS13C | 2481 | QESS*LFTLTFVPYGYTEVASVPVAR | 5 |
| −1.6 | −1.2 | −5.2 | −1.1 | 400,265 | 53.3 | Mtap1a | MAP1A | §1794 | VPSAPGQESPVPDT*KSTPPTR | 2 |
| −1.6 | −1.2 | −5.2 | −1.1 | 400,265 | 53.3 | Mtap1a | MAP1A | 1796 | VPSAPGQESPVPDTKS*TPPTR | 2 |
| −1.6 | −1.2 | −5.2 | −1.1 | 400,265 | 53.3 | Mtap1a | MAP1A | §1797 | VPSAPGQESPVPDTKST*PPTR | 31 |
| −1.6 | −2.1 | −1.4 | −1.4 | 2,098,225 | 24.4 | Kif1a; Kif1a | KIF1A iso3 | §1537; §1540 | SRPAS*PEPELLPELDSK | 22 |
| −1.4 | −1.1 | −2.6 | -1.1 | 454,218 | 35.3 | Mtap1a | MAP1A | §1789, 1796 | VPSAPGQES*PVPDTKS*TPPTR | 3 |
| −1.4 | −1.1 | −2.6 | −1.1 | 454,218 | 35.3 | Mtap1a | MAP1A | §1789, §1797 | VPSAPGQES*PVPDTKST*PPTR | 43 |
| 1.5 | 2.0 | 1.5 | 1.1 | 674,946 | 30.3 | Mapre2 | RP1 | §218, §222 | SSPASKPGSTPS*RPSS*AK | 11 |
| 1.6 | 2.3 | 1.5 | 1.1 | 563,552 | 32.4 | Mapre2 | RP1 | §215, §222 | SSPASKPGS*TPSRPSS*AK | 2 |
| 1.7 | 1.8 | 2.1 | 1.3 | 12,258,948 | 25.6 | Add2 | ADD2 | §528, §532, §535 | S*RS*PS*TES*QLMSK | 26 |
| 1.7 | 2.2 | 1.9 | 1.2 | 519,692 | 28.2 | Mark1 | MARK1 | §504 | RNT*YVCER | 8 |
| 1.7 | 2.0 | 1.9 | 1.2 | 39,365 | 30.1 | Clasp1 | CLASP1 iso3 | §646, §649 | RQS*SGS*TTNVASTPSDSR | 5 |
| 2.2 | 1.2 | 4.4 | 1.0 | 1,375,910 | 82.0 | Epb4.1l1 | EPB41L1 iso2 + iso4 | §546; §545 | RLPSSPASPS*PK | 29 |