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. 2019 Jul 6;20(13):3333. doi: 10.3390/ijms20133333

Figure 4.

Figure 4

Pollen grain development and auxin accumulation in anthers of flowers from wild-type and bdx-1 mutant Arabidopsis plants. Normal pollen grain development in wild-type (wt) flowers. (A) Anthers from 7–8 stage flower. (B) Anthers from 11–12 stage flower. (C) Anthers from 13 stage flower. GFP detection of auxins in anthers from wt/DR5 flowers. (D) Anthers from 7–8 stage flower. (E) Anthers from 11–12 stage flower. (F) Anthers from 13 stage flower. Throughout the development of the anther there is an important accumulation of auxins. Pollen grain development in bdx-1/DR5 flowers. (G) Anthers from 7–8 stage flower. (H) Anthers from 11–12 stage flower. (I) Anthers from 13 stage flower. The development of pollen grains is altered; at the 11–12 stage, diffuse cytoplasm is observed and, at stage 13, some pollen grains have collapsed. GFP detection of auxins in anthers from bdx-1/DR5 flowers. (J) Anthers from 7–8 stage flower. (K) Anthers from 11–12 stage flower. (L) Fertilized flowers at stage 13. Throughout the development of the anther there is almost no accumulation of auxins. The Arabidopsis transgenic plants were obtained as described in Cruz-Valderrama et al. [32]. Sections: 1–2 μm. Staining was with toluidine blue. Red bar = 20 μm, Black bar = 10 μm. n = 8–10 plants for each line. White bar = 100 μm. n = 5–9 anthers from flowers from different plants.