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Absorption spectra for the produced variants of P450 BM3 in their ferrous carbon monoxide complex. A typical absorption spectrum for the WT of P450 BM3 is shown in blue. EV—empty vector (negative control), WT—wild-type, GS2 (R255S/P329H/F331L), and GS3 (I122V/R255S/P329H/F331L).
