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. 2019 Jul 17;10:798. doi: 10.3389/fphar.2019.00798

Table 1.

Primers designed for qRT-PCR validation of candidate miRNAs, mRNAs, circRNAs and lncRNAs.

Forward primer Reverse Primer Tm (°C) Product length
β-actin 5′GTGGCCGAGGACTTTGATTG3′ 5′CCTGTAACAACGCATCTCATATT3′ 60 73
U6 5′GCTTCGGCAGCACATATACTAAAAT3′ 5′CGCTTCACGAATTTGCGTGTCAT3′ 60 89
hsa-miR-125b-2-3p 5′GGGGTCACAAGTCAGGCTCT3′ 5′GTGCGTGTCGTGGAGTCG3′ 60 64
hsa-miR-338-3p 5′GGGGGTCCAGCATCAGTGA3′ 5′GTGCGTGTCGTGGAGTCG3′ 60 65
hsa-miR-320b 5′AGCTGGGTTGAGAGGGCAA3′ 5′GTGCGTGTCGTGGAGTCG3′ 60 57
hsa-miR-5096 5′GGGGAGTTTCACCATGTTG3′ 5′CAGTGCGTGTCGTGGAGTC3′ 60 66
LRG1 5′GGAGCAGACAGCGACCAAA3′ 5′CAGCAGCAGCAGGAACAGAG3′ 60 74
AZGP1 5′GGAGACCCTGAAAGACATCGTG3′ 5′AACCAAACCTTCCCTGCAATAC3′ 60 74
TRIM29 5′ATGCGCCACGTTGAGAAGAT3′ 5′CGAGGGCTGGTATGATGTCC3′ 60 204
APOBEC3G 5′CATCGTGACCAGGAGTATGAGG3′ 5′AAGTAGTAGAGGCGGGCAACA3′ 60 137
2:206841107|206881891 5′GAGCAAGCAGATGAGGCAAAG3′ 5′GGTGATGTGACAATCCAGGTAGC3′ 60 126
2:206866697|206881891 5′AGGCAAAGCACACTCTGAAGC3′ 5′GGGAACATCTGGCAGGTCTAA3′ 60 116
ENST00000313807 5′TCAAATCTCCCTGGGTCTCC3′ 5′TATCCAAAGGCATCGTCTATCT3′ 60 217
MSTRG.128888.1 5′CAAAGGTCACAGACATTCAGGCA3′ 5′CCAGTTCTTACACCTTCGGTCATC3′ 60 242