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. 2019 Jul 5;20(13):3315. doi: 10.3390/ijms20133315

Figure 3.

Figure 3

Alu overexpression from lentivirus vector stably inserted in the genome. The bar plots report the fold increase of AluSq2 and AluSx expression in either (left panel) IMR90 or (right panel) HeLa cells that were stably transformed with a lentivirus vector carrying the corresponding Alu. The fold increase was derived from comparisons with cells transformed with the empty vector (background Alu expression). Alu RNA levels were quantified by RT-qPCR analysis, conducted with primers chosen to target unique sequence tracts within the Alu 3′ trailer region. In all measurements, gene expression levels were normalized to U1 gene expression, used as an internal standard.