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H2O2 accumulation and cell death detection in ZH8015 and lmm24. (A) Evans blue staining for cell death. (B) Diaminobenzidine (DAB) staining for H2O2 accumulation. (C) DNA fragmentation detection in mesophyll cells by TUNEL assay. Red signal represents staining with propidium iodide, and green signals indicate TUNEL-positive nuclei of dead cells. Scale bar:1 cm in (A,B), 50 μm in (C).
