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. 2019 Jul 2;20(13):3243. doi: 10.3390/ijms20133243

Figure 4.

Figure 4

Enhanced resistance in the lmm24 to M. oryzae. (A) Spraying method to infect ZH8015 and lmm24 at seedling stage (20 dps), leaves were photographed at 7 dpi. (B) Punch inoculation method to infect ZH8015 and lmm24 at tillering stage (50 dps), leaves were photographed at 12 dpi. (C) Relative lesion area of the inoculated leaves of ZH8015 and lmm24 plants at seedling stage (7 dpi). Data are means ± SE of 10 plants (Student’s t-test: **p < 0.01) (D) Relative Lesion area of the inoculated leaves of ZH8015 and lmm24 plants at tillering stage (12 dpi). Data are means ± SE of 10 plants (Student’s t-test: *p < 0.01). (E) Expression levels of PR genes in leaves of ZH8015 and lmm24 plants at seedling stage (20 dps). β-actin1 as a reference gene. (F) Expression levels of PR genes in leaves of ZH8015 and lmm24 plants at tillering stage (50 dps), β-actin1 as a reference gene. the expression level of each gene in ZH8015 was normalized to 1. Data are means ± SE of three biological replicates. The p-value is calculated by the Mann–Whitney U test method. *p < 0.01. Scale bar: 3 cm in (A), 2 cm in (B).