Figure 5.

Candidate region and structure of LMM24. (A) SNP index Manhattan plot of chromosome 3. Candidate gene sites are shown in the red circle. (B) Structure and mutation site of lmm24. Four exons and three introns are indicated by blue rectangles and black lines. Two C to T point and 54 bp insertion mutation were identified in the fourth exon. Sequence analysis of the mutation site in plants of ZH8015 and lmm24. (C) Agarose gel electrophoresis using a molecular marker at the insertion site. Agarose is 2%. The fragment corresponding to ZH8015 is the size of the PCR product without insertion mutation. The fragment corresponding to lmm24 is the size of the PCR product with the insertion mutation.