Figure 2.

Protein levels of glycolytic enzymes and OXPHOS in CML cells. (A,B) Protein levels of HKII and LDH-A were assessed by western blot analysis of whole cell lysates from K562 (A) and KCL-22 (B) cells exposed to 0.1, 0.5, 1 μM imatinib or vehicle for 24 or 48 h. (C) Levels of p-PKM2^Tyr105, p-PKM2^Ser37 along with total form of PKM2 and PKM1 after 48 h treatment with imatinib assessed by western blotting. (D) Levels of OXPHOS in K562 and KCL-22 cells exposed to 0.1, 0.5 or 1 μM imatinib at 48 h. Actin served to ensure equal loading. At least three independent assays were performed.