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Nb119 binds to F4/80+ CD11b+ MHCIIintermediate peritoneal macrophages. The peritoneal cavity cells from B7 naive mice were gated according to MHCII and CD11b expression. MHCIIintermediate and CD11b+ population shows F4/80 positive and Nb119 positive. F4/80 low MHCIIhigh population is Nb119 negative. Flow cytometry histogram plots of APC-labeled Nb119 (blue) and are shown in comparison with an APC-labeled rat IgG2a kappa isotype control antibody (red).
