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. 2019 Jul 6;20(13):3329. doi: 10.3390/ijms20133329

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Histogram of luciferase activity shown by HeLa cells after treatment with some of the most active compounds compared with untreated cells and with Fluc used as positive control; (B) Immunofluorescence of IB3.1 cells untreated (Untr; negative control) or treated with PTC124 (Ataluren; positive control) and compounds 1–5 respectively. Cystic fibrosis transmembrane regulator (CFTR) protein was revealed by a specific antibody targeting its first external loop (secondary antibody in green, Alexa-488). Nuclei (blue) were DAPI (4′,6-diamidino-2-phenylindole) stained.