Table 1.
Regression equation, linear range, lower limit of quantification (LLOQ), and upper limit of quantification (ULOQ) of five alkaloids in cell lysates by LC–MS/MS analysis. RE: relative error.
| Compound | Regression Equation | r | Linear Range (ng/mL) | LLOQ | ULOQ | ||
|---|---|---|---|---|---|---|---|
| Found Concentration (ng/mL) | RE (%) | Found Concentration (ng/mL) | RE (%) | ||||
| Protopine | y = 1.6558x + 0.0078 | 0.9992 | 0.75–30 | 0.728 | −2.9 | 28.62 | −4.6 |
| Chelidonine | y = 4.2789x + 0.0087 | 0.9987 | 0.5–20 | 0.495 | −1.0 | 20.48 | 2.4 |
| Coptisine | y = 1.1156x + 0.0399 | 0.9992 | 12.5–500 | 13.35 | 6.8 | 533.5 | 6.7 |
| Sanguinarine | y = 0.1732x + 0.0784 | 0.9989 | 20.0–800 | 20.72 | 3.6 | 770.4 | −3.7 |
| Chelerythrine | y = 0.2818x − 0.0068 | 0.9949 | 6.0–240 | 5.508 | −8.2 | 252.7 | 5.3 |