Table 3.
Extraction recovery and matrix effect of five alkaloids and IS in cell lysates for LC–MS/MS analysis.
| Compound | Nominal Concentration | Extraction Recovery | Matrix Effect | ||
|---|---|---|---|---|---|
| (ng/mL) | Mean (%) | RSD (%) | Mean (%) | RSD (%) | |
| Protopine | 1.875 | 108.7 | 13.4 | 93.5 | 12.1 |
| 6.0 | 90.2 | 8.0 | 99.9 | 3.1 | |
| 22.5 | 96.3 | 10.1 | 107.2 | 3.0 | |
| Chelidonine | 1.25 | 98.3 | 7.7 | 89.4 | 1.6 |
| 4.0 | 90.8 | 9.4 | 101.7 | 4.4 | |
| 15.0 | 101.1 | 9.4 | 106.7 | 2.6 | |
| Coptisine | 31.25 | 88.7 | 5.2 | 91.8 | 2.1 |
| 100 | 81.3 | 8.3 | 96.4 | 10.3 | |
| 375 | 96.1 | 8.5 | 107.1 | 7.0 | |
| Sanguinarine | 50 | 88.2 | 10.1 | 87.5 | 1.5 |
| 160 | 90.0 | 9.1 | 98.1 | 2.8 | |
| 600 | 99.6 | 7.4 | 105.9 | 2.7 | |
| Chelerythrine | 15 | 98.6 | 12.1 | 90.7 | 1.8 |
| 48 | 92.9 | 3.4 | 104.2 | 2.9 | |
| 180 | 92.1 | 10.9 | 106.7 | 3.3 | |
| Palmatine (IS) | 32 | 92.6 | 8.9 | 100.5 | 5.5 |