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. 2019 Jun 28;24(13):2393. doi: 10.3390/molecules24132393

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Optimization of the KG/PEP ratio in different KG phosphorylation reactions with ATP regeneration. (A) Reactions were performed in 1 mL of Tris-HCl buffer 50 mM and 50 μmol of the limiting substrate at different ratios of KG/PEP (1.0/0.5 (●), 1.0/0.7 (○), and 1.0/0.9 (▼), 1.0/1.1 (∆)) and a control reaction with no ATP (■). (B) Reactions were performed in 2 mL of Tris-HCl buffer 50 mM and 40 μmol of the limiting substrate at different ratios of KG/PEP (1.0/0.5 (●) and 1.0/0.7 (○)) and a control reaction with no ATP (■). (C) Reactions were performed in 2.5 mL of Tris-HCl buffer 50 mM and 35 μmol of the limiting substrate employing a KG/PEP ratio of 1.0/0.8.