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Several SA analogs consistently strengthen the interactions between citrus NPR1 and NPR3 proteins in a Y2H system. Yeast strains were incubated for 24 h in double dropout liquid media before being washed in sterile deionized water, diluted, and plated on quadruple dropout agar media with or without 200 μM SA or SA analogs. Plates were incubated at 30 °C for 72 h. QD is quadruple dropout –Leu–Trp–His–Ade. DD is double dropout–Leu–Trp.
