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. 2019 Jul 5;12(13):2157. doi: 10.3390/ma12132157

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence analyses. (A) Undifferentiated hPDLSCs showed a negative expression for CD31. (B) Undifferentiated hPDLSCs showed a negative expression for CD34. (C) Undifferentiated hPDLSCs showed a slightly positive expression for Vascular Endothelial Growth Factor-A (VEGF-A). (D) E-hPDLSCs showed a strong positive expression for CD31. (E) E-hPDLSCs showed a strong positive expression for CD34. (F) E-hPDLSCs showed a strong positive expression for VEGF-A. Scale bar: 10 µm. Magnification: 20×. Green fluorescent: cytoskeleton actin; Blue fluorescent: nuclei; Red fluorescent: specific markers. Cytofluorimetric analysis of the expression of CD31 and CD34 in hPDLSCs and in E-hPDLSCs confirm the immunofluorescence qualitative results.