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. 2019 Jul 5;20(13):3300. doi: 10.3390/ijms20133300

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CCN3-induced suppression of miR-608 enhances Runx2 and osterix expression. (A) Open-source software (miRWalk, miRanda, and Targetscan) was utilized to identify microRNAs (miRNAs) that could possibly interfere with Runx2 and osterix transcription. (B) Osteoblasts were incubated with CCN3 for 24 h and miR-608 expression levels were examined by qPCR assay. Osteoblasts were transfected with miR-608 mimic and subsequently stimulated with CCN3. mRNA and protein expression of Runx2 and osterix were examined by qPCR (C) and Western blot assay (D), respectively. Results are expressed as the mean ± S.E. * p < 0.05 as compared with the control group; # p < 0.05 as compared with the CCN3-treated group.