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. 2019 Jul 5;20(13):3313. doi: 10.3390/ijms20133313

Figure 1.

Figure 1

Impact of myoblast-conditioned medium or TGFβ signaling at mouse tissue-derived stromal cells (ADSCs). (A) ADSC morphology at 1 (24 h), 3, and 7 day of culture in control (CTRL), myoblast-conditioned (CM), and supplemented either with TGFβ (TGFb) or antibody against TGFβ (TGFb Ab) medium. (B) Expression of mRNAs encoding CD90, CD73, and PDGFRα. Expression was related to the levels observed in cells cultured in control medium at day 1, and normalized to the level of mRNA encoding β-actin. RQ: relative quantity. (C) ADSC growth curve at 1 (24 h), 3, and 7 day of culture in control, or myoblasts-conditioned, supplemented either with TGFβ or antibody against TGFβ medium. (D) In vitro scratch wound healing assay—cells were scratched from culture dish and the area which was not invaded by migrating cells was presented (at 6 h and 24 h). For each time point or experimental group n ≥ 3. Data are presented as mean ± SD. *represent results of Student’s t-test: * p ≤ 0.05; ** p ≤ 0.01, **** p ≤ 0.0001.