Figure 3.

Evaluation of H₂O₂ treatment and exposure time needed for reactive oxygen species (ROS) generation and oxidative stress-induced ARPE-19 cell death. (A) Undifferentiated and (B) differentiated ARPE-19 cells were treated with different concentrations of H₂O₂ (within the range 100–500 µM) over a time course of 0–6 h after which cell viability was measured using MTT assay. ROS production was also measured under the same experimental conditions for both (C) undifferentiated and (D) differentiated ARPE-19 cells. Graphs represent average cell viability and relative ROS production (mean ± SD values, n = 4; One-Way ANOVA test, * p ≤ 0.05 vs. control group).