Fig. 1.

Apigenin has mild antitumor effects on cancer cells with activating EGFR mutation. a H1975, HCC827, H1650, H3255 and SK-MEL-28 cells were treated with increasing doses of apigenin (Apg) for 2 days. Cell viability was assessed using MTT assay. IC50 was analyzed by GraphPad Prism 7.00. b Cells were seeded in 24-well plates and treated with increasing doses of Apg as indicated for 14 days. The adherent cells were then stained with crystal violet for the colony formation assay (left). Colony formation ability was analyzed (right)