Fig. 4.

ABT-263 and apigenin significantly suppressed the growth and proliferation of AZD9291-resistant H1975 cells. a H1975 parental and H1975 AZD9291-resistant (H1975-AR) cells were treated with increasing doses of AZD9291 for 2 days. Cell viability was assessed using MTT assay. b H1975 and H1975-AR cells were seeded in 24-well plates and treated with increasing doses of AZD9291 for 21 days. The adherent cells were then stained with crystal violet for the colony formation assay (left) and colony formation ability was analyzed (right). c H1975 and H1975-AR cells were seeded in 24-well plates and were treated with Apg (15 µM) and ABT (2 µM), alone or comb for 1 days, cell viability rates were examined by MTT. d H1975-AR cells were seeded in 24-well plates and treated with Apg (15 µM) and ABT (2 µM), alone or comb for 14 days for the colony formation assay (upper), colony formation ability was analyzed (lower). The data represents mean ± SD (n = 3). *p < 0.05, and **p < 0.01