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. 2019 Jul 23;20:27. doi: 10.1186/s12860-019-0209-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — The effect of simultaneous silencing Stat1 and FoxO1 on apoptosis and ROS level. The experiments were divided into six groups, which were control, HG (30 μM), siStat1 + HG (Stat1 siRNA + 30 μM HG), siStat1 + siFoxO1 + HG (Stat1 siRNA + FoxO1 siRNA + 30 μM HG), siFoxO1 + HG (FoxO1 siRNA + 30 μM HG) and siFoxO1 (FoxO1 siRNA). a Apoptosis levels were detected by flow cytometry. b Relative apoptosis rate showed as bar diagrams. c ROS level showed as bar diagrams. d ROS levels were detected by flow cytometry. Data were expressed as mean ± SD from three independent experiments. (*Compared with control, ^# Compared with siStat1 + HG, */^#P < 0.05, **/^##P < 0.01)