Fig. 7.

Silencing Stat1 promotes the expression of antioxidant genes in high glucose injured MPC-5 cells. a The Catalase, MnSOD, NQO1 and HO1 protein levels were detected by western blot in MPC-5 cells. b Relative protein expression of Catalase showed as bar diagrams. c Relative protein expression of MnSOD showed as bar diagrams. d Relative protein expression of NQO1 showed as bar diagrams. e Relative protein expression of HO1 showed as bar diagrams. f mRNA expression of Catalase was assessed using qRT-PCR in MPC-5 cells. g mRNA expression of MnSOD was assessed using qRT-PCR in MPC-5 cells. h mRNA expression of NQO1 was assessed using qRT-PCR in MPC-5 cells. i mRNA expression of HO1 was assessed using qRT-PCR in MPC-5 cells. GAPDH served as an internal control. Data were expressed as mean ± SD from three independent experiments. (*Compared with control, ^#Compared with HG, */^#P < 0.05, **P < 0.01)