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. 2019 Jul 23;20:27. doi: 10.1186/s12860-019-0209-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — The effect of over-expressing FoxO1 on the expression of antioxidant genes, Stat1 and FoxO1. The experiments were divided into three groups, which were control, Mock + HG and over-expressing FoxO1 + HG. a The protein expression of FoxO1 in nucleus and cytoplasm, respectively, Lamin B1 served as a nucleus loading control, Tubulin served as a cytoplasm internal control. b Relative protein expressions of FoxO1 in nucleus and cytoplasm showed as bar diagrams. c The Catalase, MnSOD, NQO1, HO1, Stat1 and FoxO1 protein levels were detected by western blot in MPC-5 cells. d Relative protein expression of Catalase, MnSOD, NQO1, HO1, Stat1 and FoxO1 showed as bar diagrams. e mRNA expression of Catalase, MnSOD, NQO1, HO1, Stat1 and FoxO1 were assessed using qRT-PCR in MPC-5 cells. GAPDH served as an internal control. Data were expressed as mean ± SD from three independent experiments. (*Compared with control, ^# Compared with Mock + HG, */^#P < 0.05, **/^##P < 0.01)