Table 2.
Kinetic parameters of all the enzymes in the n-butanol pathway
| Enzyme | Substrate for Km measurements | Km (mM) | Vmax (µmol/min/mg)a | kcat/Km (103 M−1s−1) |
|---|---|---|---|---|
| Tt_Thl | Acetyl-CoA | 2.4 ± 0.58b | 19.2 ± 1.8 | 11.7 ± 3.5 |
| Tt_Hbd | Acetoacetyl-CoA | 0.03 ± 0.01 | 67.4 ± 7.4 | 3955 ± 658 |
| Tt_Crt | Hydroxybutyryl-CoA | 0.6 ± 0.1 | 181.3 ± 15.4 | 607 ± 54 |
| St_Ter | Crotonyl-CoA | 0.03 ± 0.01 | 22.6 ± 2.2 | 924 ± 89 |
| Ts_Bad | Butyryl-CoA | 0.2 ± 0.1 | 16.1 ± 2.2 | 79.1 ± 1.5 |
| Ts_Bdh | Butyraldehyde | 4.2 ± 0.9 | 27.2 ± 3.4 | 9.1 ± 0.9 |
| Ts_Bad | Acetyl-CoA | 0.09 ± 0.01 | 5.2 ± 0.7 | 71.1 ± 6.9 |
| Ts_Bdh | Acetaldehyde | 5.8 ± 0.8 | 31.2 ± 3.1 | 7.6 ± 0.3 |
a0.3 mM NADH was used as cofactor
bError represents one standard deviation, n = 3