Table 6.

Strain used in this work

Organism	Strain	Description	Accession numbera	Source or reference
C. thermocellum	LL1004	Wild-type C. thermocellum strain DSM 1313	CP002416	DSMZ
	AG929	DSM1313 Δhpt ΔClo1313_0478	SRP097241	[8]
	LL1644	Strain AG929 Δldh with gene Tt_thl,Tt_hbd, Tt_crt, St_ter,Ts_bad and Ts_bdh integrated in the genome	SRP190757	This work
	LL1669	Strain LL1644 ΔClo1313_1353-1356	SRP190758	This work
	LL1668	LL1669 with pLT_229	SRP190756	This work
E. coli	T7 Express lysY/lq	Used for heterologous protein expression		New England Biolabs
	DH5α	Used for plasmid screening and propagation		New England Biolabs

^aFor strains with sequenced genomes, this is the GenBank accession number. For re-sequenced strains, this is the Sequence Read Archive (SRA, https://www.ncbi.nlm.nih.gov/sra) accession number