Table 7.
Plasmids used in this work
| Plasmid | Descriptiona | GenBank accession number |
|---|---|---|
| pDGO143 | Gene expression plasmid for C. thermocellum [8] | KX259110 |
| BT01 | pDGO143 with Tt_thl, Tt_hbd, Tt_crt, Ts_ bcd, Ts_etfAB and Ca_adhE2 | MK524015 |
| BT02 | pDGO143 with Tt_thl, Tt_hbd, Tt_crt, Ts_ bcd, Ts_etfAB, Ts_bad and Ts_bdh | MK524016 |
| BT03 | pDGO143 with Tt_thl, Tt_hbd, Tt_crt, Ts_ bcd, Ts_etfAB, Ts_bad and Ca_bdh | MK542521 |
| BT04 | pDGO143 with Tt_thl, Tt_hbd, Tt_crt, St_ter and Ca_adhE2 | MK542522 |
| BT05 | pDGO143 with Tt_thl, Tt_hbd, Tt_crt, St_ter, Ts_bad and Ts_bdh | MK542523 |
| BT06 | pDGO143 with Tt_thl, Tt_hbd, Tt_crt, St_ter, Ts_bad and Ca_bdh | MK542524 |
| BT07 | pDGO143 with Re_phaAB, Ac_phaJ, Ts_ bcd, Ts_etfAB and Ca_adhE2 | MK542525 |
| BT08 | pDGO143 with Re_phaAB, Ac_phaJ, Ts_ bcd, Ts_etfAB, Ts_bad and Ts_bdh | MK542526 |
| BT09 | pDGO143 with Re_phaAB, Ac_phaJ, Ts_ bcd, Ts_etfAB, Ts_bad and Ca_bdh | MK542527 |
| BT10 | pDGO143 with Re_phaAB, Ac_phaJ, St_ter and Ca_adhE2 | MK542528 |
| BT11 | pDGO143 with Re_phaAB, Ac_phaJ, St_ter, Ts_bad and Ts_bdh | MK542529 |
| BT12 | pDGO143 with Re_phaAB, Ac_phaJ, St_ter, Ts_bad and Ca_bdh | MK542530 |
| pD861-CH | E. coli rhamnose-inducible expression vectors from ATUM (Newark, CA) | |
| pLT_181 | pD861-CH with Tt_thl gene | MK542531 |
| pLT_182 | pD861-CH with Tt_thlM1 gene | MK542532 |
| pLT_183 | pD861-CH with Tt_thlM2 gene | MK542533 |
| pLT_190 | pD861-CH with Tt_hbd gene | MK542534 |
| pLT_193 | pD861-CH with Tt_crt gene | MK542535 |
| pLT_194 | pD861-CH with St_ter gene | MK542536 |
| pLT_195 | pD861-CH with Ts_bad gene | MK542537 |
| pLT_196 | pD861-CH with Ts_bdh gene | MK542538 |
| pLT_164 |
Integrate Ts_bad and Ts_bdh to C. thermocellum genome C. thermocellum enolase promoter was used for gene expression [32] |
MK542539 |
| pLT_191 |
Integrate Tt_thl and Tt_hbd to C. thermocellum genome C. thermocellum Clo1313_2638 promoter was used for gene expression [32] |
MK542540 |
| pLT_199 |
Integrate Tt_crt and St_ter to C. thermocellum genome C. thermocellum enolase promoter was used for gene expression [32] |
MK542541 |
| pJGW37 | Gene expression plasmid for C. thermocellum | [46] |
| pLT_208 | pJGW37 with Tt_thlM1 | MK542542 |
| pLT_209 | pJGW37 with Tt_thlM2 | MK542543 |
| pLT_228 | pJGW37 with Tt_thlM2, Tt_hbd and St_ter | MK542544 |
| pLT_229 | pJGW37 with Tt_thlM2, Tt_hbdM and St_terM | MK542545 |
aGenes are listed in the 5′–3′ direction in their operon