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. 2019 Jul 9;2019:3427053. doi: 10.1155/2019/3427053

Figure 3.

Figure 3

DHI modulated Th1/Th2 immune responses in a mouse model of CS-induced inflammation. Percentage of CD4+IFN-γ+ Th1 cells in the HLN (a, c) and spleen (b, d) was detected by flow cytometry (n = 5 to 6 per group). (e) ELISA analysis of IFN-γ levels in the BALF (n = 5 per group). (f, g) Th1 nuclear transcription factor T-bet expression levels in mouse lung and spleen were assayed by qRT-PCR (n = 5 to 6 per group). (h, i) ELISA analysis of IL-4 and IL-13 levels in the BALF (n = 5 per group). (j) Th2 nuclear transcription factor GATA3 expression levels in mouse lung were assayed by qRT-PCR (n = 5 to 6 per group). Data are presented as the mean ± SEM. ∗P < 0.05; ∗∗P < 0.01. All experiments were performed in triplicate.