DUX4 activity: p38 inhibitors do not block DUX4 transcriptional activation function. Non-DUX4 expressing 54-6 (normal) myoblasts were transfected with a DUX4 expression plasmid and treated with varying concentrations of control p300 inhibitor A-485 (A), p38 inhibitor PH-797804 (B) or p38 inhibitor pamapimod (C), as indicated, for 20 hours. Cultures were analyzed for DUX4 targets (MBD3L2, ZSCAN4, and LEUTX) RNA levels by quantitative real-time PCR. Data are expressed as relative expression with the expression in absence of inhibitor set to 100.