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. 2019 Aug;370(2):219–230. doi: 10.1124/jpet.119.259663

Fig. 5.

Fig. 5.

p38 Inhibitors suppress DUX4 expression in a mouse xenograft pharmacology model of FSHD gene regulation. (A) PH-797804 was administered to xenograft mice twice daily by subcutaneous injections at the indicated doses for 4 days. RNA was isolated from excised tibialis anterior (TA) muscles and analyze for DUX4, MBD3L2, ZSCAN4, and LEUTX RNA levels. (B) Losmapimod was administered orally to xenograft mice twice daily at the indicated doses for 4 days. RNA was isolated and analyzed as in (A). (C) Losmapimod was administered orally to xenograft mice twice daily at 6 mg/kg for 14 days. RNA and DNA were isolated from excised TA muscles and analyzed for differentiation marker MYH2 RNA levels and two-copy gene (hTERT and mTfrc) DNA levels. Data are expressed as relative expression (mean with S.E.) with the expression in the vehicle groups set to 1. For MYH2 analysis, the 14-day levels were compared with levels in 4-day animals (day 4) not treated with drugs, which was set to 1. *P < 0.01 vs. vehicle (one-way ANOVA with Dunnett’s post test). #P = 0.030 vs. vehicle (unpaired two-tailed t test).