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. 2019 Jul 4;20(13):3293. doi: 10.3390/ijms20133293

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PtoABCG36 enhances cadmium tolerance in yeasts. (A) Δyap1 and the wild-type Y252 were transformed with EV (pDR196 empty vector) and pDR196-PtoABCG36, and grown on SD plates with indicated concentrations of CdCl₂ for 7 d. (B,C) Growth curves of yeast cells Δyap1-EV (square), Δyap1- PtoABCG36 (circle), Y252- EV (up-triangle) and Y252- PtoABCG36 (down-triangle) under control (B) and 40 µM CdCl₂ condition (C) for indicated time. (D) Accumulation of cadmium in Δyap1-EV (navy blue), Δyap1-PtoABCG36 (purple), Y252-EV (dark cyan) and Y252-PtoABCG36 (orange) yeasts. Yeast cells (1 × 10⁷) were exposed to 40 µM Cd treatment for 6, 12, 18 or 24 h at 30 °C. Cd concentrations in the yeast cells were measured by ICP-OES. Error bars indicate standard deviation (n = 3). Different letters indicated significant differences (p < 0.05).