Table 1.
The results of the profiling of Se/GSH-depleted rats.
| Parameters | Group | Week 2 | Week 4 | Week 5 |
|---|---|---|---|---|
| Serum Se level (μg/L) | Control | 509.0 ± 34.8 | 550.0 ± 34.8 | 607.0 ± 69.7 |
| Se/GSH (−) | 355.0 ± 4.6 ** | 396.0 ± 56.0 * | 412.0 ± 79.7 * | |
| Hepatic GSH level (μmol/g liver) | Control | 2.90 ± 0.10 | 3.03 ± 0.06 | 3.53 ± 0.35 |
| Se/GSH (−) | 5.37 ± 0.97 * | 5.80 ± 0.89 ** | 1.43 ± 0.47 ** | |
| Hepatic GSSG level (μmol/g liver) | Control | 0.95 ± 0.15 | 1.44 ± 0.56 | 1.47 ± 0.22 |
| Se/GSH (−) | 0.16 ± 0.04 ** | 0.11 ± 0.03 * | 0.61 ± 0.33 * | |
| GSSG/GSH ratio | Control | 0.33 ± 0.01 | 0.47 ± 0.17 | 0.41 ± 0.17 |
| Se/GSH (−) | 0.03 ± 0.01 ** | 0.02 ± 0.002 * | 0.41 ± 0.10 | |
| Hepatic MDA level (×10−2 μmol/g liver) | Control | 13.7 ± 0.3 | 12.6 ± 0.4 | 12.2 ± 0.5 |
| Se/GSH (−) | 13.4 ± 0.1 | 14.4 ± 0.4 ** | 14.3 ± 0.4 ** | |
| Plasma AST level (U/L) | Control | 75.7 ± 1.5 | 57.3 ± 6.5 | 59.0 ± 7.5 |
| Se/GSH (−) | 65.7 ± 8.6 | 60.0 ± 57.3 | 55.7 ± 1.5 | |
| Plasma ALT level (U/L) | Control | 25.7 ± 4.0 | 18.3 ± 2.5 | 22.3 ± 7.4 |
| Se/GSH (−) | 21.0 ± 3.0 | 21.0 ± 1.7 | 17.3 ± 3.2 | |
| Plasma T-BIL level (mg/dL) | Control | 0.047 ± 0.006 | 0.053 ± 0.021 | 0.053 ± 0.025 |
| Se/GSH (−) | 0.043 ± 0.006 | 0.050 ± 0.010 | 0.053 ± 0.021 | |
| Plasma GLDH level (U/L) | Control | 18.3 ± 1.2 | 16.7 ± 2.3 | 15.7 ± 2.9 |
| Se/GSH (−) | 14.7 ± 2.9 | 17.0 ± 4.4 | 15.0 ± 2.6 | |
| Plasma SDH level (U/L) | Control | 1.17 ± 0.76 | 2.17 ± 1.44 | 4.33 ± 1.53 |
| Se/GSH (−) | 1.67 ± 1.15 | 2.17 ± 2.47 | 4.00 ± 2.00 |
Mean ± S.D. are presented with 5 determinations. * p < 0.05, ** p < 0.01 (Student’s t-test). Se: Selenium, GSH: Glutathione, GSSG: Glutathione disulfide, MDA: Malondialdehyde, AST: Aspartate aminotransferase, ALT: Alanine aminotransferase, T-BIL: Total-bilirubin, GLDH: Glutamate dehydrogenase, SDH: Sorbitol dehydrogenase, Se/GSH (−): Se/GSH-depleted group.