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Nε(carboxymethyl)-lysine (CML) contents and % amino acid residue glycation in AGE-BSA molecules with different incubation time.
| Incubation Time of AGE | Nε(carboxymethyl)-Lysine (μg/mL) | Number(%) of Glycation in AGE | ||||
|---|---|---|---|---|---|---|
| Exp. 1 | Exp. 2 | Exp. 3 | Lysine | Arginine | Cysteine | |
| Day 1 | 0.056 | 0.049 | — | — | — | — |
| Day 30 | 0.136 | 0.269 | 0.458 | 43/56(77%) | 2/23(8%) | 0/35(0%) |
| Day 45 | 1.126 | 0.171 | 0.186 | — | — | — |
| Day 130 | 7.586 | 7.197 | 7.779 | 56/56(100%) | 1/23(4%) | 0/35(0%) |
| Day 180 | 10.916 | 10.664 | 9.509 | 56/56(100%) | 4/23(16%) | 0/35(0%) |
