Fig. 3.

The impact of mutation of bcsA₃₉₃₇ and ycgR₃₉₃₇ on various virulence phenotypes were examined. Bacterial swimming motility (A), biofilm formation (B) and pectate lyase production (C) were measured in the parental strain D. dadantii 3937, ΔbcsA₃₉₃₇, ΔegcpB, ΔegcpBΔbcsA₃₉₃₇, ΔecpC and ΔecpCΔbcsA₃₉₃₇ respectively. The same assays were also tested in the parental strain 3937, ΔycgR₃₉₃₇, ΔegcpB, ΔegcpBΔycgR₃₉₃₇, ΔecpC and ΔecpCΔycgR₃₉₃₇ (D–F). Assays were performed as described in Experimental procedures. The experiments were repeated three independent times with similar results. The figure represents results from one experiment which includes three to five technical replicates. Error bars indicate standard errors of the means. Asterisks indicate statistically significant differences of the means (P < 0.05 by Student’s t-test).