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. Author manuscript; available in PMC: 2019 Jul 24.
Published in final edited form as: Environ Microbiol. 2015 Oct 14;17(11):4745–4763. doi: 10.1111/1462-2920.13029

Fig. 4.

Fig. 4.

The impact of mutation of bcsA3937 and ycgR3937 on hrpA promoter activity was examined. (A) The hrpA promoter activity was measured in the parental strain D. dadantii 3937, ΔbcsA3937, ΔegcpB, ΔegcpBΔbcsA3937, ΔecpC and ΔecpCΔbcsA3937 respectively. Cells cultured under T3SS-inducing condition were used to measure the mean fluorescence intensity (MFI) by flow cytometry. The same assays were performed in the parental strain 3937, ΔycgR3937, ΔegcpB, ΔegcpBΔycgR3937, ΔegcpB ycgR3937R124D, ΔecpC, ΔecpCΔycgR3937 and ΔecpC ycgR3937R124D (B). The experiments were repeated three independent times with similar results. The figure represents results from one experiment which includes three technical replicates. Error bars indicate standard errors of the means. Asterisks indicate statistically significant differences of the means (P < 0.05 by Student’s t-test).