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. Author manuscript; available in PMC: 2020 Jun 18.
Published in final edited form as: Anal Chem. 2019 May 31;91(12):7578–7585. doi: 10.1021/acs.analchem.8b05889

Figure 2.

Figure 2.

Optimization of trypsin concentration for microLESA digestion. (A) A section of rat liver was divided into 3 regions. For each microLESA extract collected (purple circle outline), 3 separate trypsin microdigests were performed (red filled circle). Each concentration was tested in triplicate, extracting digested peptides from the 3 microdigested spots. (B) The number of unique proteins identified as a function of trypsin concentration increased as the concentration increased. (C) The overlap in protein identifications is depicted for each trypsin concentration, with the majority of the proteins identified in the 0.75 and 7.5 ng/μL samples being found in the 75 ng/μL samples.