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. Author manuscript; available in PMC: 2020 Jun 18.
Published in final edited form as: Anal Chem. 2019 May 31;91(12):7578–7585. doi: 10.1021/acs.analchem.8b05889

Figure 4.

Figure 4.

Autofluorescence microscopy and MALDI IMS images of fluorescently labeled S. aureus infected mouse kidney tissue. (A) Autofluorescence microscopy images were acquired to visualize the abscess and bacterial colonies targeted for digestion. The interrogated abscess is circled in purple and a “zoomed-in” capture highlights the colonies that were targeted for digestion (red circles) using the microLESA workflow. (E) MALDI FT-ICR IMS was used to investigate the distribution of intact proteins present in the tissue. Selected proteins are presented that were identified using the data collected from the microLESA experiment, correlating the identifications by accurate mass matching.