Skip to main content
. Author manuscript; available in PMC: 2019 Jul 24.
Published in final edited form as: Trends Genet. 2018 Sep 27;35(1):3–5. doi: 10.1016/j.tig.2018.09.003

Figure 1. Improving estimates of cis-trans divergence.

Figure 1.

a. Estimating trans-acting divergence from the difference between parental expression and hybrid ASE. Parental divergence is estimated from the ratio of parental expression levels, and is assumed to be the product of cis and trans effects (additive in log-space). Figure adapted from [14]. b. The standard method of cis-trans comparison leads to artifactual negative correlation when cis estimates have any error. Note “REP1” could represent either a single replicate or an average of multiple replicates. c. The proposed method of cross-replicate comparison is not inflated by random measurement error. d. Histogram showing the difference between the two methods applied to the same data set [4]. Each cis-trans correlation is based on one pair of hybrid/parental replicates (36 pairs for the standard method and 90 for cross-replicate comparison, each with over 4,000 informative genes).