Figure 4. Macrophage presence and differentiation in aortic tissue.
At day 7 after aneurysm induction (d14 after transfection with MC) aortic tissue was harvested, lysed and analyzed via flow cytometry to characterize macrophage differentiation markers. A, B, D include representative flow cytometry graphs and histograms; n=4/5/4
A) Percentage of TNF-α+ of F4/80+ cells in untreated, AngII-infused, and AngII-infused/MC-IL10 transfected animals; * = p≤0.05 vs. AngII MC-IL10, # = p≤0.05 vs. untreated.
B) Percentage of IL-10+ of F4/80+ cells; ** = p≤0.01 vs. AngII
C) Ratio of TNF-α+ over IL-10+ cells; * = p≤0.05 vs. AngII MC-IL10, # = p≤0.05 vs. untreated.
D) Percentage of MRC1+ of F4/80+ cells; * = p≤0.05 vs. AngII