Figure 4.

(A) Scatterplot illustrating the relationship between the vitamin D dose–response in the differentiated versus less differentiated cluster of cells. Red denotes genes up or downregulated in a similar fashion in both groups. Encircled blue are a subset of genes, all of which were significantly downregulated upon vitamin D stimulation in more differentiated cells, but were instead upregulated in cells from the less differentiated cluster (FDR for interaction <0.05). (B) A ridgeplot illustrating gene-set enrichment analysis (GSEA) of the genes with opposite/contrasting differential expression pattern in more versus less differentiated cells. Boxplots of genes with a reversed expression pattern in more versus less differentiated cells illustrating a clear anti-differentiation effect of vitamin D where cells with high expression of genes involved in muscle differentiation respond with a robust downregulation of these genes, (C) miR-133, (D) MSTN (Myostatin) and (E) RYR1.