FIGURE 3.

Schematic representation of fusion-constructs and transient expression assays in N. benthamiana leaves. (A) Scheme of mCherryFP and mGFP genes under the bidirectional promoter (BP) and confocal stacks spanning tobacco epidermal cells of transient transformed leaves at 3 and 4 post-infiltration days (dpi). Projections are from mCherryFP (a,d,i,l), mGFP (c,f,g,j) and the corresponding Nomarski snap-shots (b,e,h,k). Scale bars represent 50 μm. (B) Relative expression levels (2^–dCt) of mCherryFP and mGFP genes in agroinfiltrated N. benthamiana leaves with the control vector (pmdc107ø) or with the constructs including the bidirectional promoter fused to the two reporter genes. Data are means ± SE of three replicates. Different letters indicate significant differences (P < 0.05 Wald χ² followed by LSD test). Primers used for this experiment are listed in Supplementary Table 1.