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. 2019 Jul 17;10:930. doi: 10.3389/fpls.2019.00930

FIGURE 4.

FIGURE 4

Schematic representation of fusion-constructs and expression assays in stably transformed Arabidopsis plants in response to T. urticae infestation. (A) Scheme of mCherryFP and mGFP genes under the bidirectional promoter (BD) and confocal stacks spanning Arabidopsis epidermal cells of transformed leaves under control conditions, and after T. urticae infestation. Projections are from mCherryFP (b,d,f,h,i,k,m,o,r,t) and mGFP (a,c,e,g,j,l,n,p,q,s). Bars are 50 μm. (B) Quantitative analyses of mGFP and mCherryFP gene expression in stably transformed Arabidopsis plants in control, and after 24 h of T. urticae infestation. Bars in green and red colors correspond to mGFP and mCherryFP gene expression, respectively. Data are ±SE of three biological replicates. Different letters indicate statistical differences (P < 0.05 Wald χ2 followed by LSD test). Arabidopsis transformed lines for mGFP::BP:mCherryFP construct were 1.1 and 2.4, and for mCherryFP:BP::mGFP were 1.3 and 4.6. Primers used for this experiment are listed in Supplementary Table 1.