Figure 1.

Impact of CRP on BON1 cells. (A) IL-6 secretion of BON1 cells significantly increased upon CRP exposure (20 µg/mL, 48 h, ELISA, *P < 0.01). (B) Migration/invasion assay model: BON1 cells seeded in the inserts pass through basement membrane and cling to the bottom of insert’s membrane. Invasive cells are counted with nine individual fields per membrane. Blue dots = BON1 cells, clear circles = membrane pores. (C and D) Compared to normal non-treated BON1 cells, significantly more BON1 cells were capable of invading the gel and migrating through the membrane after CRP treatment (invasion assay, *P < 0.0001). (E) Proliferation capability of BON1 cells was strongly enhanced in the presence of CRP as well as IL-6 in MTS assay (*P < 0.001). (F) However, this effect cannot be seen in BrdU assay.