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. 2019 Jul 16;25:5289–5298. doi: 10.12659/MSM.915682

Figure 2.

Figure 2

Down-regulation of testis-specific gene antigen 10 (TSGA10) increased bladder cancer cell apoptosis in vitro. (A) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay for mRNA expression of TSGA10 and Western blot show the level of TSGA10 in 5637 cells transfected with pcDNA3.1 or pcDNA3.1-TSGA10, siRNA, or siTSGA10. (B) Flow cytometry analysis of cell apoptosis and the percentage of total apoptotic cells show that TSGA10 inhibits apoptosis in 5637 cells. (C) Western blot assay shows the effects of down-regulation or upregulation of TSGA10 on 5637 cells and apoptosis protein expression levels, including cleaved caspase-3, cleaved caspase-9, and p53. The data are presented as the mean ± standard deviation (SD). * p<0.05 and ** p<0.01.