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. 2019 Jul 16;25:5280–5288. doi: 10.12659/MSM.915038

Figure 3.

Figure 3

Apigenin activated the Nrf2-HO-1 signaling pathway in high glucose-induced cells. HK-2 cells were treated with 30 mM D-glucose with or without apigenin (100, 200 μM) for 48 h. (A) qRT-PCR showed that apigenin enhanced mRNA expression of Nrf2 in HK-2 cells treated with 30 mM D-glucose. (B) Western blot showed that apigenin enhanced protein expression of Nrf2 in HK-2 cells. (C). LY294002 pretreatment significantly increased Nrf2 protein levels compared to HK-2 cells with D-glucose. (D) Apigenin also enhanced mRNA expression of HO-1. *** P<0.001 vs. HG group. HG – high glucose.