FIG 2.
Kinetics of proliferation of R. anatipestifer CH-1 and R. anatipestifer ATCC 11845 inside G. mellonella larvae and ducklings. (A) G. mellonella larvae were infected with 106 CFU of CH-1 and ATCC 11845. After 2, 12, 24, 36, and 48 h, the bacterial load in larvae was recorded. Six live larvae per time point were killed for these assays. (B) Three-day-old ducklings were infected with 108 CFU of CH-1 and ATCC 11845. After 24 and 48 h, the bacterial loads in the blood, liver, and spleen were recorded. Six ducklings at the time point were killed for these assays. These results were repeated at least three times. Each error bar represents 1 SD from the mean. Differences were assessed for statistical significance with one-way analysis of variance (ANOVA).