FIG 5.

The ΔphiA mutant has a higher multiplication rate. (A) Growth curve in TSB of B. abortus wild-type and ΔphiA mutant strains over 28 h. (B) OD₆₀₀ and CFU at 20 h of the growth curve. *, P < 0.0001; **, P < 0.05. (C) Bright-field microscopy images of the B. abortus wild-type and ΔphiA mutant strains grown in TSB at 24 h of the growth curve. The average size (100 bacteria measured) of the bacteria is indicated in each image. (D) Growth curve in TSB of B. abortus wild-type and ΔphiA mutant strains over 16 h. In this curve, the generation time of each strain was calculated (inset table). (E) Growth curve in TSB of B. abortus wild-type, ΔphiA mutant, and complemented strains over 18 h. (F) OD₆₀₀ at 15 h of the growth curve. *, P < 0.05.